超净工作台标准使用方法及日常维护

    本文着重对超净工作台的定义，超净工作台的运行原理，应用方法及超净工作台的日常使用维护做一个详细说明！国内外读者均可以方便阅读本文！

   一、什么是超净工作台呢？

　　超净工作台是一种局部净化设备，即利用空气净化技术使一定操作区内的空间达到相对的无尘、无菌状态。使用时，必须放置在洁净的空间。其接种数量不受无菌空的限制，操作比较简便，可以较大幅度地提高工作效率，适用于大规模生产。为了保证接种成功率，接种时使用酒精灯，效果更好。

    Clean Bench is a partial purification equipment, namely the use of air purification technology to a certain region of space operations to achieve a relatively dust-free, sterile condition. Used, must be placed in a clean space. The number of its vaccination is not sterile space constraints, the operation is simpler, can be more greatly improve work efficiency, suitable for mass production. In order to ensure the success rate of vaccination, inoculation using alcohol lamp, better.

　　二、超净工作台运作原理及应用方法

　　超净工作台的优点是操作简单自如，较为舒适，工作效率高，预备时间短，开机十分分钟以上即可操作，基本上可随时使用。在工厂化生产中，接种工作量很大，需要经常长久地工作时，超净台是很理想的设备。超净台由三相电机作鼓风动力，功率145～260W左右，将空气通过由特制的微孔泡沫塑料片层叠合组成的“超级滤清器”后吹送出来，形成连续不断的无尘无菌的超净空气层流，即所谓“高效的特殊空气”，它除去了大于0.3μm的尘埃、真菌和细菌孢子等等。超净空气的流速为24～30m/min，这已足够防止附近空气可能袭扰而引起的污染，这样的流速也不会妨碍采用酒精灯或本生灯对器械等的灼烧消毒。工作人员就在这样的无菌条件下操作，保持无菌材料在转移接种过程中不受污染。但是万一操作中途遇到停电，暴露在未过滤空气中的材料便难以幸免污染。这时应迅速结束工作，并在瓶上作出记号，内中的材料如处于增殖阶段，则以后不再用作增殖而转入生根培养。如为一般性生产材料，因极其丰富也可弃去。如处于生根过程，则可留待以后种植用。

    Clean Bench has the advantage of easy to operate freely, more comfortable, efficient, preparation time is short, the boot can be operated for more than a very minute, basically ready to use. In factory production, inoculation heavy workload, the need for frequent long-term work, the Clean Bench is the ideal equipment. Clean Bench by the three-phase motor to make blast power, power 145 ~ 260W or so, will air through the porous foam by a specially composed piece cascading together a "super filter" after blowing out the formation of a continuous dust-free The ultra-clean air laminar flow bacteria, so-called "high-performance special air", which removed more than 0.3μm dust, fungi and bacteria spores and so on. Ultra-clean air flow rate of 24 ~ 30m/min, which are adequate to prevent possible harassment in the vicinity of air pollution caused by such a flow will not impede the use of alcohol lamp or Bunsen burner burning disinfection of equipment and so on. And in this way the staff operated under sterile conditions to maintain sterile material in the course of the transfer of vaccination is not contaminated. But what if an electrical outage halfway operation, exposed to unfiltered air contaminated material will not be spared. Should provide a speedy end to work and make a mark on the bottle, which is in the materials, such as the proliferation phase, we will no longer be used as a proliferation and transferred to rooting culture. As for the general production of materials, due to the extremely rich can also be disposable. As in the rooting process, can be planted until later use.

　　超净工作台电源多采用三相四线制，其中有一零线，连通机器外壳，应接牢在地线上，另外三线都是相线，工作电压是380V。三线接入电路中有一定的顺序，如线头接错了，风机会反转，这时声音正常或稍不正常，超净台正面无风(可用酒精灯火焰观察动静，不宜久试)，应及时切断电源，只要将其中任何两相的线头交换一下位置再接上，就可解决。三相线如只接入两相，或三相中有一相接触不良，则机器声音很不正常，应立即切断电源仔细检修，否则会烧毁电机。这些常识应在开始使用超净台时就向工作人员讲解清楚，免除不应造成的事故与损失。

    Clean Bench power supply to use more three-phase four-wire system, of which 10 lines, connecting the machine casing should then firmly on the ground line, the other three lines are the phase, the working voltage is 380V. Third-line access to the circuit in a certain order, such as pick the wrong end of a thread, wind the opportunity to reverse, when the sound normal or slightly abnormal, Clean Bench front without wind (available alcohol lamp flame observation of static and dynamic, not long-trial), should be promptly cut off the power, as long as any of them as two-phase end of a thread to exchange position were followed, the will be over. Three-phase line, such as access only to two-phase or three-phase there is a similar connection is bad, then the machine sounds very normal, should be immediately cut off the power supply careful maintenance, otherwise they will burn motor. These common sense should be when they start using Clean Bench to explain clearly to staff, should not result in exemption from the accident and loss.

　　超净工作台进风口在背面或正面的下方，金属网罩内有一普通泡沫塑料片或无纺布，用以阻拦大颗粒尘埃，应常检查、拆洗，如发现泡沫塑料老化，要及时更换。除进风口以外，如有漏气孔隙，应当堵严，如贴胶布，塞棉花，贴胶水纸等。工作台正面的金属网罩内是超级滤清器，超级滤清器也可更换，如因使用年久，尘粒堵塞，风速减小，不能保证无菌操作时，则可换上新的。

    Clean Bench at the back or front air inlet below the metal mesh cover within a normal foam piece or non-woven fabrics used to block large particles of dust, should be a routine check, unpick and wash, such as found in foam aging, must be promptly replaced. In addition to outside air inlet, where gas leakage pores, it should be blocked strict, such as patching, plug of cotton and paste glue paper. Table inside the front cover of the metal mesh filter is super, super filter may be replaced, as a result of the use of long years, dust jam, wind speed decreased, can not guarantee aseptic operation, can be replaced with new.

    超净台使用寿命的长短与空气的洁净程度有关。在温带地区超净台可在一般实验室使用，然而在热带或亚热带地区，由于大气中含有高量的花粉，或多粉尘的地区，超净台则宜放在较好的有双道门的室内使用。任何情况下不应将超净台的进风罩对着开敞的门或窗，以免影响滤清器的使用寿命。

    Clean Bench length of life related to the degree of air cleanliness. Clean Bench in temperate regions may be in the general laboratory use, but in tropical or subtropical regions, because the atmosphere contains high levels of pollen, dust or more areas, Clean Bench are advised to put a better interior double doors. Any case, Taiwan should not be ultra-clean air inlet hood facing the open door or window, so as not to affect the filter life.

　　无菌室应定期用70%酒精或0.5%苯酚喷雾降尘和消毒，用2%新洁尔灭抹拭台面和用具(70%酒精也可)，用福尔马林(40%甲醛)加少量高锰酸钾定期密闭熏蒸，配合紫外线灭菌灯(每次开启15分钟以上)等等消毒灭菌手段，以使无菌室经常保持高度的无菌状态。接种箱内部也应装有紫外线灯，使用前开灯15分钟以上照射灭菌，但凡是照射不到之处仍是有菌的。在紫外线灯开启时间较长时，可激发空气中的氧分子缔合成臭氧分子，这种气体成分有很强的杀菌作用，可以对紫外线没有直接照到的角落产生灭菌效果。由于臭氧有碍健康，在进入操作之前应先关掉紫外线灯，关后十多分钟即可入内。

    Sterile room should be on a regular basis with 70% alcohol or 0.5% phenol spray dust and disinfected with 2% wiped countertops and appliances Bromogeramine (70% alcohol can also be), with formalin (40% formaldehyde) plus a small amount of high manganese airtight fumigation potassium on a regular basis to meet the ultraviolet sterilization lamps (each to open more than 15 minutes) and so on means of disinfection and sterilization, sterile rooms in order to maintain regular high level of sterility. Vaccination should also be equipped with a UV box inside lights, turn on the lights more than 15 minutes prior to use radiation sterilization, but not of the Department any exposure there is still bacteria. UV light to open longer, it can stimulate oxygen molecules in the air to enter the ozone molecule synthesis, this gas composition has a strong bactericidal effect, you can not directly shine on the corner of ultraviolet sterilizing effect produced. Because ozone unhealthy, on entering the operation should be preceded by turn off the UV lamp off after more than 10 minutes you can enter.

　　在超净工作台上亦可吊装UV紫外线灯，但应装在照明灯罩之外，并错开照明灯平行排列，这样在工作时不妨碍照明。若将紫外线灯装入照明灯罩(玻璃板)里面，这是毫无用处的，因为紫外线不能穿透玻璃，它的灯管是石英玻璃，而不是硅酸盐玻璃制成的。

     In the ultra-clean the table can also be hoisting UV ultraviolet light, but it should be packed in light shade outside, and stagger the lights arranged in parallel, so that at work, without prejudice to lighting. Ruoqiang ultraviolet light into light shade (glass) inside, which is useless, since ultraviolet light can not penetrate the glass, it is a quartz glass tube, rather than made of silicate glass.

　　接种室内力求简洁，凡与本室工作无直接关系的物品一律不能放入，以利保持无菌状态。接种室内的空气与外界空气应绝对隔绝，预留的通气孔道应尽量密闭。通气孔道可设上下气窗，气窗面积宜稍大，需覆上4层纱布作简单滤尘。在一天工作之后，可开窗充分换气，然后再予以密闭。总之，既要清洁无尘无菌，又要空气新鲜，适宜工作。覆在通气窗上的纱布应经常换洗。但是上述种种措施只是理想的设计方案，往往不易全面做到，其实只要严格无菌操作手续，在门窗敞开的室内，有一超净台的保护，接种的污染率仍可控制在生产上可以容忍的水平。

　　三、超净工作台的日常维护

    Third, the daily maintenance of ultra-clean bench

　　超净工作台是一台较精密的电气设备，对其进行经常性的保养和维护是非常重要的。

    我们怎样做好保养净化设备的最重要的超净工作台工作呢？

　　首先要保持室内的干燥和清洁，潮湿的空气既会使制造材料锈蚀，还会影响电气电路的正常工作，潮湿空气还利于细菌、霉菌的生长。清洁的环境还可延长滤板的使用寿命。

　　另外，定期对设备的清洁是正常使用的重要环节。清洁应包括使用前后的例行清洁和定期的熏蒸处理。熏蒸时，应将所有缝隙完全密封。清洁应包括使用前后的例行清洁和定期的熏蒸处理。熏蒸时，应将所有缝隙完全密封，如操作口设有可移动挡板封盖的类型超净工作台，可用塑料薄膜密封。

　　超净工作台的滤板和紫外杀菌灯都有标定的使用年限，应按期更换。

 补充一点：在使用超净工作台的工程中，要注意不要有相关的震动产品，会影响到工作台内部的风机使用寿命！

相关产品：垂直流超净工作台  产品所有：http://www.fenglins.com 苏小姐：13570963006